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KMID : 0380219990320020140
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Journal of Biochemistry and Molecular Biology
1999 Volume.32 No. 2 p.140 ~ p.146
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Comparison of TNF-Mediated Glucose Catabolism between the TNF-Sensitive and -Resistant Cell Lines
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Kim Yeon-Hyang
Park Bok-Ryun
Cheong Hee-Sun
Kwon Oh-Hwan
Kim Dae-Que
Kim Soung-Soo
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Abstract
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When murine fibrosarcoma L929 cells, a TNF-sensitive cell line, were treated with recombinant human tumor necrosis factor-α (rhTNF-α), the activities of glycolytic regulatory enzymes and lactate dehydrogenase increased up to 100-150% compared to the control L929 cells after TNF treatment. By using various metabolic inhibitors and activators, it was found that cAMP-dependent protein kinase is responsible for the increase of activities of the glycolytic enzymes. The activities of glycolytic regulatory enzymes and lactate dehydrogenase of TNF-resistant A549 cells, a human lung carcinoma cell line, did not increase significantly compared to TNF-sensitive L929 cells upon TNF treatment. In contrast, the pyruvate carboxylase activities of A549 cells, but not L929 cells, increased up to 30~40% after TNF treatment. The data suggest that pyruvate carboxylase activity may contribute to the compensation of energy loss mediated by TNF treatment in TNF-resistant A549 cells.
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KEYWORD
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cAMP, Glucose uptake, Glycolytic enzyme, Lactate dehydrogenase, Pyruvate carboxylase, Tumor necrosis factor
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